5 ^{Upstream processing for}

viral vaccines–General

aspects

Lars Pelz and Sven Göbel

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany

Karim Jaen

Department of Internal Medicine II, Klinikum rechts der

Isar, Technical University of Munich, Munich, Germany and

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany

Udo Reichl

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany and

Chair for Bioprocess Engineering, Otto-von-Guericke-

University Magdeburg, Magdeburg, Germany

Yvonne Genzel

Bioprocess Engineering, Max Planck Institute for Dynamics

of Complex Technical Systems, Magdeburg, Germany

CONTENTS

5.1

Introduction...................................................................................................81

5.2

Basic Design of a Virus Production Process ..............................................81

5.3

Vaccine Candidates......................................................................................83

5.4

Cell Lines for Virus Production ..................................................................87

5.4.1 Selection of Host Cell Lines..........................................................87

5.4.2 Cell Banking...................................................................................90

5.4.3 Preculture and Maintenance of Cells.............................................91

5.4.4 Adherent Versus Suspension Cells................................................92

5.4.4.1 Cell Attachment and Use of Microcarriers ....................94

5.5

Cultivation Media and Metabolism .............................................................95

5.5.1 Media and Additives......................................................................95

5.5.2 Monitoring Cell Metabolism........................................................100

DOI: 10.1201/9781003229797-5

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